| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jessop, Autumn G (12)
|
MAUN |
28.70 |
28.16 |
-0.54 |
| 2 |
Incollingo, Rachel D (13)
|
MAGAAC |
29.06 |
29.05 |
-0.01 |
| 3 |
Skalicky, Reagan A (13)
|
MAGAAC |
29.85 |
29.60 |
-0.25 |
| 4 |
Skalicky, Reese M (13)
|
MAGAAC |
29.68 |
29.67 |
-0.01 |
| 5 |
Abraham, Evelyn V (13)
|
MACHEL |
29.88 |
29.73 |
-0.15 |
| 6 |
Amons, Molly E (13)
|
MACHEL |
31.83 |
30.93 |
-0.90 |
| 7 |
Sileo-Magliari, Sophia (10)
|
MAUDAC |
33.14 |
33.19 |
0.05 |
| 8 |
Cosgrove, Scarlett J (11)
|
MAGAAC |
33.51 |
33.80 |
0.29 |
| 9 |
McGillis, Isabela L (13)
|
MACHEL |
35.03 |
34.04 |
-0.99 |
| 10 |
Gonnella, Ava R (10)
|
MAGAAC |
36.63 |
34.13 |
-2.50 |
| 11 |
Tooze, Grace P (9)
|
MAEAAC |
35.82 |
36.41 |
0.59 |
| 12 |
Abraham, Audrey (11)
|
MACHEL |
37.74 |
38.26 |
0.52 |
| 14 |
Gonnella, Lila G (9)
|
MAGAAC |
|
41.66 |
- |
|
McCrudden, Reiley M (13)
|
MAGAAC |
31.41 |
30.34 |
-1.07 |
|
Baughman, Caroline E (9)
|
MAGAAC |
38.57 |
38.82 |
0.25 |